Product : Bromophenol Blue
C.A.S. No. :115-39-9
M.F. :C19H10Br4O5S
M.W. :669.99gm/mole
Specifications:
Dye Content (by Spectrophotometry) >95%
Transition Range pH 3.0-4.6
Greenish Yellow-Blue
Solubility 0.1% (95% EtOH) Clear solution
Absorption maximum (pH 3.0) ?1max 434-439nm
Absorption maximum (pH 4.6) ?2max 590-593nm
Absorptivity (A1%/1cm at pH 3.0 at ?1max) 350-385
Absorptivity (A1%/1cm at pH 4.6 at ?2max) 940-1000
Loss On Drying (110�°C) <1%
Product Description:
Bromophenol blue is an acid-base indicator since its useful range lies between pH 3.0 and 4.6. It changes from yellow at pH 3.0 to blue at pH 4.6; this reaction is reversible. Bromophenol blue is structurally related to phenolphthalein (a popular indicator). Bromophenol blue is also used as a color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis.