Number of Reactions(Preps)/Kit : 96 Wells
Brand : CTK BioTech
Sample : serum / plasma
Stroage : 2-8 C
Cat No : AE0410
INTENDED USE:
The TRUSTwell HIV 1+2 Ab ELISA Kit is a solid phase enzyme linked immunosorbent assay for the qualitative detection of anti-HIV-1 including subtype O and anti-HIV-2 antibodies (including isotype IgG, IgM and IgA) in human serum or plasma. It is intended for professional use only as an aid in the diagnosis of infection with HIV-1 and HIV-2 viruses. Any reactive specimen with the TRUSTwell HIV 1+2 Ab ELISA Kit must be confirmed with alternative testing method(s) and clinical findings.
TEST PRINCIPLE :
TRUSTwell HIV 1+2 Ab ELISA Kit is a solid phase enzyme linked immunosorbent assay based on the principle of the double antigen-sandwich technique for the detection of the various antibodies against HIV-1 and/or HIV-2 in human serum or plasma. The TRUSTwell HIV 1+2 Ab ELISA Kit is composed of two key components: 1) Solid microwells pre-coated with recombinant HIV-1 and HIV-2 antigens; 2) Liquid conjugates composed of recombinant HIV-1 and HIV-2 antigens conjugated with horse radish peroxidase (HIV 1+2 HRP conjugates). During the assay, the test specimen is first incubated with the coated microwells. The anti-HIV-1 and anti-HIV-2 antibodies, if present in the specimen, bind to the antigens coated on the microwell surface. In the second incubation with the HRP- HIV 1+2 conjugates, the anti-HIV-1 and antiHIV-1 antibodies absorbed on the surface of microwell react to the HRP-HIV 1+2 conjugates. Unbounded conjugates are then removed by washing. The presence of the complexed conjugates is shown by a blue color upon additional incubation with TMB substrate. The reaction is stopped with Stop Solution and absorbance are read using a spectrophotometer at 450 /620-690 nm.
Clinical Performance :
A total of 1095 patient specimens from susceptible subjects were tested by the TRUSTwell HIV 1+2 Ab ELISA Kit.
Number of Reactions(Preps)/Kit : 96 Wells
Brand : CTK BioTech
Sample ; Serum
Storage : 2-8 C
Cat No : E1030
The RecombiLISA TSH ELISA is a solid phase enzyme linked immunosorbent assay for the quantitative determination of the levels of Thyroid Stimulating Hormone (TSH) in human serum.
The RecombiLISA TSH ELISA is intended for professional use as an aid in the diagnosis of thyroid dysfunction. TSH values should not be the only factor in the determination of thyroid status, and all other available clinical and epidemiological information should be considered for an accurate diagnosis.
Recommended for use:
Any person presenting with signs or symptoms of hypo or hyperthyroidism
Any person presenting with an enlarged thyroid gland (goiter)
Pregnant women for first trimester screening
Any person requiring regular monitoring of treatment
Normally, thyroid function is first assessed by detection of TSH levels in the blood.
The monoclonal anti-TSH antibody coated on the surface of the microwells specifically recognizes the junction between the �± and �² subunits. The HRP-conjugated monoclonal anti-TSH antibody detects the �² subunit only.
Combining the measurement of TSH with the thyroid hormones (T3/T4) can provide more information for the determination of thyroid status.
Product Features :
Sensitivity: Detects TSH levels as low as 0.035 �µIU/mL
Specificity: No cross reactivity detected with related hormones: FSH, LH and hCG
Linearity: 1:128 dilution on 36 IU/mL sample
Precision: intra assay precision: CV 7.5%; inter assay precision: CV 8.0 %
Accuracy: R2= 0.986 against Roche TSH Chemiluminescence Immunoassay
Hook effect: No hook effect up to 8000 IU/mL
Interference: not affected by Hemoglobin 500mg/dL, Diltiazem 15uM, Bilirubin 20 mg/dL, Erythromycin 81.6uM, Triglycerides 3g/dL, Verapamil 4.4uM, HAMA 52.5ng/mL, T3 100ng/mL, RF 215IU/mL, T4 100ug/dL, Acetaminophen 1324uM
Calibration: Human TSH WHO 3rd International Standards (81/565 NIBSC)
Calibrators: 0, 0.5, 2.0, 5.0, 10, 20, 40 �µIU/mL in biological matrix
Shelf life: 18 months
Assay time: 80 minutes.
Product Specifications:
Anti-TSH Ab coated microwells
TSH standard S1
TSH standard S2
TSH standard S3
TSH standard S4
TSH standard S5
TSH standard S6
TSH standard S7
HRP-anti-TSH conjugates
TMB substrate
Wash buffer (30X concentrate)
Stop solution
ELISA working sheet
Product insert
Number of Reactions(Preps)/Kit : 96 Wells
Brand : CTK BioTech
Sample : serum / plasma
Storage : 2 - 8 C
Cat No : AE0710
INTENDED USE:
The TRUSTwell HBsAg ELISA Kit is a solid phase enzyme linked immunosorbent assay for the qualitative detection of hepatitis B virus surface antigen (HBsAg) in human serum or plasma. It is intended for professional use only as an aid in the diagnosis of infection with HBV. Any reactive specimen with the TRUSTwell HBsAg ELISA Kit must be confirmed with alternative testing method(s) and clinical findings.
TEST PRINCIPLE:
TRUSTwell HBsAg ELISA Kit is a solid phase enzyme linked immunosorbent assay based on the principle of antibody sandwich technique for the detection of HBsAg in human serum or plasma. The TRUSTwell HBsAg ELISA Kit is composed of two key components: 1) Solid microwells pre-coated with monoclonal anti HBsAg antibody; 2) Liquid conjugates composed of polyclonal anti-HBsAg conjugated with horse radish peroxidase (HRP-HBsAb conjugates). During the assay, the test specimen and HRP-HBsAb conjugates are incubated simultaneously with the coated microwells. HBsAg, if present in the specimen, reacts to the anti-HBsAg antibody coated on the microwell surface as well as the HRPHBsAb conjugate, forming sandwich complex conjugates. Unbounded conjugates are then removed by washing. The presence of the complexed conjugates is shown by a blue color upon additional incubation with TMB substrate. The reaction is stopped with Stop Solution and absorbance are read using a spectrophotometer at 450 /620-690 nm.
Clinical Performance:
A total of 1033 patient specimens from susceptible subjects were tested by the TRUSTwell HBsAg ELISA Kit.
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Florfenicol in samples. The coupling antigens are pre-coated on the micro-well stripes. The Florfenicol in the sample and the conjugate antigens pre-coated on the micro-well stripes compete for the anti-Florfenicol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value has a negative correlation with the Florfenicol concentration in the sample. This value is compared to the standard curve and the Florfenicol concentration is subsequently obtained.
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Gentamicin in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Gentamicin in the sample and pre-coated coupling antigen on the micro-well stripes compete for the anti-Gentamicin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Gentamicin in it. The value is compared to the standard curve and the Gentamicin concentration is subsequently obtained.
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Tylosin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Tylosin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Tylosin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tylosin in it. This value is compared to the standard curve and the Tylosin concentration is subsequently obtained.
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of chloramphenicol in the tissue (chicken, pork), honey, milk, fish, shrimp and egg. The coupling antigen is pre-coated on the micro-well stripes. The chloramphenicol in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes for the antibody against chloramphenicol. After the addition of the enzyme conjugate, the tmb substrate is added for coloration. The optical density (od) value of the testing sample has a negative correlation with the content of chloramphenicol in it. This value is compared to the standard curve and the content of the corresponding chloramphenicol is subsequently obtained.
