In enzymology, a dtmp kinase (ec 2.7.4.9) is an enzyme that catalyzes the chemical reaction: atp + dtmp rightleftharpoons adp + dtdp. Thus, the two substrates of this enzyme are atp and dtmp, whereas its two products are adp and dtdp. This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with a phosphate group as acceptor. This enzyme participates in pyrimidine metabolism.
Tyrosine is an important nutritionally essential amino acid that plays an important role in the metabolism, growth and development of humans and animals, and is widely used in food, feed, medicine and chemical industries. It is often used as a nutritional supplement for patients with phenylketonuria, and as a raw material for the preparation of pharmaceutical and chemical products such as polypeptide hormones, antibiotics, L-dopa, melanin, p-hydroxycinnamic acid, and p-hydroxystyrene. As more high-value-added L-tyrosine derivatives such as danshensu, resveratrol, and hydroxytyrosol are found in organisms, L-tyrosine is increasingly developing in the direction of platform compounds.
Tyrosine is one of the 22 kinds of amino acids used by cells to synthesize proteins. It can be used to synthesize proteins in cells. Its codons are UAC and UAU. It is a non-essential amino acid that contains polar side groups and can be synthesized by the human body. The word "tyrosine" comes from the Greek tyros, meaning cheese. It was first discovered in the casein of cheese by the German chemist Justus von Liebig in the early 19th century. When it is used as a functional group or a side group, it is called tyrosyl.
Function
In addition to being a proteinogenic amino acid, tyrosine has a special role in signal transduction in proteins by means of a phenolic function, which functions as a receptor for phosphate groups transferred by protein kinases (so-called tyrosine kinase receptors). organ, while phosphorylation of hydroxyl groups alters the activity of the target protein.
Tyrosine also plays an important role in photosynthesis. In chloroplasts (photosystem II), it is used as an electron donor in the reduction reaction of oxidized chlorophyll, allowing it to deprotonate the phenolic OH-group, and finally in Photosystem II is reduced by four core manganese clusters.
Dietary Sources
Tyrosine can be synthesized from phenylalanine in the body and can be found in many high-protein foods such as chicken, turkey, fish, milk, yogurt, cheese, cottage cheese, peanuts, almonds, pumpkin seeds, sesame, soybeans, lima beans , found in avocados and bananas.
The Casein kinase 1 family of protein kinases are serine/threonine-selective enzymes that function as regulators of signal transduction pathways in most eukaryotic cell types. CK1 isoforms are involved in Wnt signaling, circadian rhythms, nucleo-cytoplasmic shuttling of transcription factors, DNA repair, and DNA transcription.
Product Name: D-Fructose 1,6-bisphosphate trisodium salt
Molecular Formula: C6H15NaO12P2
Molecular Weight: 364.11
Appearance: White to Off-white powder
Purity: 99%
CAS NO.: 38099-82-0
EINECS No.: 253-778-0
Supplier: ZHENYIBIO
Fructose-1,6-biphosphate (F1,6P) is a glycolytic intermediate produced by the transfer of a phosphate from ATP to fructose-6-phosphate by the enzyme phosphofructokinase. Fructose-1,6-biphosphate, along with fructose-2,6-biphosphate, modulates the activity of phosphofructokinase-1 (PFK-1), the rate-limiting step in glycolysis. During glycolysis, aldolase splits Fructose-1,6-biphosphate into dihydroxacetone phosphate (DHAP) and glyceraldehyde phosphate. Fructose-1,6-biphosphate is also an allosteric activator of the M2 isoform of Pyruvate Kinase (PK-M2), the predominant form of pyruvate kinase in cancer cells.
Tyrosine is an important nutritionally essential amino acid that plays an important role in the metabolism, growth and development of humans and animals, and is widely used in food, feed, medicine and chemical industries. It is often used as a nutritional supplement for patients with phenylketonuria, and as a raw material for the preparation of pharmaceutical and chemical products such as polypeptide hormones, antibiotics, L-dopa, melanin, p-hydroxycinnamic acid, and p-hydroxystyrene. As more high-value-added L-tyrosine derivatives such as danshensu, resveratrol, and hydroxytyrosol are found in organisms, L-tyrosine is increasingly developing in the direction of platform compounds.
Tyrosine is one of the 22 kinds of amino acids used by cells to synthesize proteins. It can be used to synthesize proteins in cells. Its codons are UAC and UAU. It is a non-essential amino acid that contains polar side groups and can be synthesized by the human body. The word "tyrosine" comes from the Greek tyros, meaning cheese. It was first discovered in the casein of cheese by the German chemist Justus von Liebig in the early 19th century. When it is used as a functional group or a side group, it is called tyrosyl.
Function
In addition to being a proteinogenic amino acid, tyrosine has a special role in signal transduction in proteins by means of a phenolic function, which functions as a receptor for phosphate groups transferred by protein kinases (so-called tyrosine kinase receptors). organ, while phosphorylation of hydroxyl groups alters the activity of the target protein.
Tyrosine also plays an important role in photosynthesis. In chloroplasts (photosystem II), it is used as an electron donor in the reduction reaction of oxidized chlorophyll, allowing it to deprotonate the phenolic OH-group, and finally in Photosystem II is reduced by four core manganese clusters.
Dietary Sources
Tyrosine can be synthesized from phenylalanine in the body and can be found in many high-protein foods such as chicken, turkey, fish, milk, yogurt, cheese, cottage cheese, peanuts, almonds, pumpkin seeds, sesame, soybeans, lima beans , found in avocados and bananas.
75 Thymidylate Kinase From Prokaryote Recombinant Suppliers
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Anhydrous Milk Fat (AMF) is a concentrated dairy product consisting of nearly pure milk fat, with a minimum fat content of 99.8%. It is produced by removing almost all water and non-fat solids from milk, cream, or butter through physical separation processes. AMF is free from additives and has a smooth texture, a golden "butter" color, and a clean, characteristic flavor.
Characteristics
- Composition: Contains at least 99.8% milk fat, with minimal moisture (0.1% maximum) and other constituents like free fatty acids and salt (0.1% maximum).
- Physical Properties: AMF is liquid at temperatures above 36�°C and solid below 16â??17�°C. It is transparent in liquid form and has a smooth appearance.
- Microbiological Standards: AMF is free from harmful microorganisms like Salmonella and Listeria, ensuring its safety for consumption.
Applications
AMF is widely used in the food industry due to its versatility:
- Recombined Dairy Products: Used to create products like milk and cream by blending with water and other ingredients.
- Chocolate and Ice Cream: Enhances texture and flavor in confectionery and frozen desserts.
- Custom Fat Blends: Fractionation of AMF allows for the creation of tailored fat products with specific functional properties.
Production Methods
AMF can be produced using two main methods:
- Direct from Cream: Continuous flow processing of fresh cream or milk.
- Reworked Butter: Processing butter to extract milk fat.
Storage and Handling
AMF is typically packed in nitrogen-filled drums to prevent oxidation and can be stored for several months at +4C. It requires less storage space compared to butter and has a longer shelf life.
Let me know if you'd like to explore its production process or applications further!
Skimmed Milk Powder (Instant / Regular) (Available IN LH/MH/HH)
You can start your day with a healthy diet like Skimmed Milk Powder, Star Food made from fresh cow milk pasteurized and concentrated under required conditions. This contains the same nutrition like the organic and fresh milk has. It is a great source of proteins and vitamins. The possible packaging of Skimmed milk powder is 10 Kgs or 25 Kgs bags.
Characteristic
White to yellowish colour, free flowing powder, typical milky taste and smell without foreign odour.
Milk Permeate Concentrate (MPC) is made from fresh pasteurized skimmed milk. By a process of ultrafiltration, the milk protein is separated from the other components. The liquid concentrate is further concentrated and dried. MPC is available with a protein between 40 % and 85 % milk protein content.
Milk protein concentrate (MPC) is any type of concentrated milk product that contains 40% to 90% milk protein. MPC defines as "any complete milk protein (casein plus lactalbumin) concentrate that is 40 percent or more protein by weight. In addition to ultrafilter milk products, the MPC classification includes concentrates made through other processes, such as blending nonfat dry Milk with highly concentrated proteins, such as casein.
Characteristic
White to yellowish color, typical milk taste and smell without foreign odour.
Applications
Nutritional and dietary products, geriatric nutritional products, recombined and processed cheese, bakery and confection products, processed meat.
Benefits
High protein low lactose ratio, MPC in cheese milk brings greater cheese yield, good heat stability, forms and stabilizes fat emulsions, has excellent solubility and dispensability, high nutritional values, low heat process ensures proteins will not denature.
Storage
Product must be stored in a cool, dry place, at temperature below 25 degree and relative humidity below 65%, product has a 2 years shelf life in the original packaging.
Packaging
Four-layer paper bag with polythene inner bag, 25kg net
This is one of the most popular creamer. Made from evaporated creamer that prepared by homogenizing a recombined mix of milk solids, edible vegetable fat and water. Thats make thuis product have rich and creamy taste
General Information - Momordica charantia (MC), a member of the Cucurbitaceae family, is also known as bitter melon, bitter gourd, balsam pear, pare, or karela. It is a widely grown and consumed vegetable in Asia, East Africa, India, and South America. The potential for MC to modulate blood glucose has received the most attention from investigators searching for natural foods or compounds that may be useful in the treatment of diabetes.
Phytochemicals - The main constituents of bitter melon which are responsible for the antidiabetic effects are triterpene, proteid, steroid, alkaloid, inorganic, lipid, and phenolic compounds. Several glycosides have been isolated from the M. charantia stem and fruit and are grouped under the genera of cucurbitane-type triterpenoids. In particular, four triterpenoids have AMP-activated protein kinase activity which is a plausible hypoglycaemic mechanism of M. charantia.
The Chagas Ab Rapid Test is a lateral flow chromatographic immunoassay for the qualitative detection of IgG anti-Trypanosoma cruzi (T. cruzi) in human serum or plasma. It is intended to be used as a screening test and as an aid in the diagnosis of infection with T. cruzi. Any reactive specimen with the Chagas Ab Rapid Test must be confirmed with alternative testing method(s) and clinical findings.
Chagas disease is an insect-borne, zoonotic infection by the protozoan T. cruzi, which causes a systemic infection of humans with acute manifestations and long term sequelae. It is estimated that 16-18 million individuals are infected worldwide, and roughly 50,000 people die each year from chronic Chagas disease (World Health Organization)1.
Buffy coat examination and xenodiagnosis used to be the most commonly methods2,3 in the diagnosis of acute T. cruzi infection. However, both methods are either time consuming or lack of sensitivity. Recently, serological test becomes the mainstay in the diagnosis of Chagasâ??s disease. In particularly, recombinant antigen based tests eliminate false-positive reactions which are commonly seen in the native antigen tests4-5.
The Chagas Ab Rapid Test is an instant antibody test which detects IgG antibodies the T. cruzi within 15 minutes without any instrument requirements. By utilizing T. cruzi specific recombinant antigen, the test is highly sensitive and specific.
The HCV Gold Rapid Screen Test (RST) is a Chromatographic immunoassay (CIA) for direct qualitative detection of antibodies to
Hepatitis C type virus (HCV) in human serum/ plasma and whole blood.
PRINCIPLE
HCV RST is a chromatographic immunoassay (CIA) for the detection of antibodies to HCV in human serum/plasma and whole blood. HCV recombinant antigens are precoated onto membrane as a capture reagent on the test region. During the test, specimen is allowed to react with the colloidal gold particles, which have been labeled with HCV recombinant antigens. If antibodies to HCV present, a pink colored band will develop on the membrane in proportion to the amount of HCV antibodies present in the specimen .Absence of this pink colored band in the test region suggests a negative result. To serve as a procedural control, a purple colored band in the control region will always appear regardless the presence of antibodies to HCV.
REAGENTS AND MATERIALS PROVIDED
1. One pouched cassette with desiccant.
2. Blood diluent in a dropper bottle, stored at 4-30°C.
3. One piece of operating instruction.
WARNING AND PRECAUTIONS
1. For in vitro diagnostic uses only.
2. All patient samples should be treated as if capable of transmitting diseases.
3. Do not interchange reagents from different lots or use test kit beyond expiration date.
4. Icteric, lipemic, hemolysed, heat treated and contaminated sera may cause erroneous results.
STORAGE
The kits should be stored at temperature 4-30°C, the sealed pouch for the duration of the shelf life (24 months).
The HCV Gold Rapid Screen Test (RST) is a Chromatographic immunoassay (CIA) for direct qualitative detection of antibodies to
Hepatitis C type virus (HCV) in human serum/ plasma and whole blood.
PRINCIPLE
HCV RST is a chromatographic immunoassay (CIA) for the detection of antibodies to HCV in human serum/plasma and whole blood. HCV recombinant antigens are precoated onto membrane as a capture reagent on the test region. During the test, specimen is allowed to react with the colloidal gold particles, which have been labeled with HCV recombinant antigens. If antibodies to HCV present, a pink colored band will develop on the membrane in proportion to the amount of HCV antibodies present in the specimen .Absence of this pink colored band in the test region suggests a negative result. To serve as a procedural control, a purple colored band in the control region will always appear regardless the presence of antibodies to HCV.
REAGENTS AND MATERIALS PROVIDED
1. One pouched cassette with desiccant.
2. Blood diluent in a dropper bottle, stored at 4-30°C.
3. One piece of operating instruction.
WARNING AND PRECAUTIONS
1. For in vitro diagnostic uses only.
2. All patient samples should be treated as if capable of transmitting diseases.
3. Do not interchange reagents from different lots or use test kit beyond expiration date.
4. Icteric, lipemic, hemolysed, heat treated and contaminated sera may cause erroneous results.
STORAGE
The kits should be stored at temperature 4-30°C, the sealed pouch for the duration of the shelf life (24 months).
The HCV Gold Rapid Screen Test (RST) is a Chromatographic immunoassay (CIA) for direct qualitative detection of antibodies to
Hepatitis C type virus (HCV) in human serum/ plasma and whole blood.
PRINCIPLE
HCV RST is a chromatographic immunoassay (CIA) for the detection of antibodies to HCV in human serum/plasma and whole blood. HCV recombinant antigens are precoated onto membrane as a capture reagent on the test region. During the test, specimen is allowed to react with the colloidal gold particles, which have been labeled with HCV recombinant antigens. If antibodies to HCV present, a pink colored band will develop on the membrane in proportion to the amount of HCV antibodies present in the specimen .Absence of this pink colored band in the test region suggests a negative result. To serve as a procedural control, a purple colored band in the control region will always appear regardless the presence of antibodies to HCV.
REAGENTS AND MATERIALS PROVIDED
1. One pouched cassette with desiccant.
2. Blood diluent in a dropper bottle, stored at 4-30°C.
3. One piece of operating instruction.
WARNING AND PRECAUTIONS
1. For in vitro diagnostic uses only.
2. All patient samples should be treated as if capable of transmitting diseases.
3. Do not interchange reagents from different lots or use test kit beyond expiration date.
4. Icteric, lipemic, hemolysed, heat treated and contaminated sera may cause erroneous results.
STORAGE
The kits should be stored at temperature 4-30°C, the sealed pouch for the duration of the shelf life (24 months).
The Leishmania IgG/IgM Rapid Test is a lateral flow immunoassay for the qualitative detection of antibodies including IgG and IgM to the subspecies of the Leishmania donovani (L. donovani), the Visceral leishmaniasis causative protozoans in human serum or plasma. This test is intended to be used as a screening test and as an aid in the diagnosis of the disease of Visceral leishmaniasis. Any reactive specimen with the Leishmania IgG/IgM Rapid Test must be confirmed with alternative testing method(s).
SUMMARY AND EXPLANATION OF THE TEST
Visceral leishmaniasis, or Kala-azar, is a disseminated infection caused by several subspecies of the L. donovani. The disease is estimated by the World Health Organization (WHO) to affect approximately 12 million people in 88 countries1. It is transmitted to humans by bites of the Phlebotomus sandflies, which acquire infection from feeding on infected animals. Though it is a disease for poor countries, in Southern Europe, it has become the leading opportunistic infection in AIDS patients2-3.
Identification of L. donovani organism from the blood, bone marrow, liver, lymph nodes or the spleen provides a definite means of diagnosis. However, these test methods are limited by the sampling method and the special instrument requirement. Serological detection of anti-L. donovani Ab is found to be an excellent marker for the infection of Visceral leishmaniasis. Tests used in clinic include: ELISA, fluorescent antibody and direct agglutination tests4-5. Recently, utilization of L. donovani specific protein in the test has improved the sensitivity and specificity dramatically6-7.
The Leishmania IgG/IgM Rapid Test is a recombinant protein based serological test, which detects antibodies including IgG, IgM and IgA to the L. Donovani. This test provides a reliable result within 10 minutes without any instrumentation requirements.
The Cytomegalovirus Rapid Test is a rapid qualitative lateral flow test designed for the quantitive detection of Cytomegalovirus (CMV) in human serum/plasma samples.
Cytomegalovirus is a herpes virus and a leading biological factor causing congenital abnormalities and complications among those who receive massive blood transfusions and immunosuppressive therapy. About half of the number of pregnant women who contract a primary infection, spread the disease to their fetus. When acquired in-utero, the infection may cause mental retardation, blindness, and/or deafness. Serological tests for detecting the presence of antibody to CMV can provide valuable information regarding the history of previous infection, diagnosis or active or recent infection, as well as in screening blood for transfusions in newborns and immuno-compromised recipients.
The Cytomegalovirus Rapid Test Device (Serum/Plasma) has been designed to detect CMV through visual interpretation of color development in the internal strip. The membrane was immobilized with antigens of CMV on the test region. During the test, the specimen is allowed to react with colored recombinant mouse anti-human IgM latex conjugates, which were precoated on the sample pad of the test. The mixture then moves on the membrane by a capillary action, and interact with reagents on the membrane. If there were enough CMV antibodies in specimens, a colored band will from at the test region of the membrane. Presence of this colored band indicates a positive result, while its absence indicates a negative result. Appearance of a colored band at the control region serves as a procedural control. This indicates that proper volume of specimen has been added and membrane wicking has occurred.
Anti-Syphilis Test is a rapid direct binding test for the visual detection of anti-syphilis antibodies
in serum as an aid in the diagnosis of syphilis infection. Test results are read visually without any
instrument. It is based on the principle of double antigen sandwich immunoassay for determination of syphilis antibodies in serum. Purified recombinant syphilis antigens are employed to identify
anti-Syphilis antibodies specifically. This one step test is very sensitive and only takes about 10 to 20 minutes.
Syphilis Test SPECIMEN COLLECTION
For serum, collect blood into a container without anticoagulant. Allow the blood to clot and separate the serum from the clot. Use the serum for testing. If the specimen cannot be tested on the day of collection, store the serum specimen in a refrigerator or freezer. Bring the specimens to room temperature before testing. Do not freeze and thaw the specimen repeatedly.
Syphilis Test PROCEDURE
Strip
1.When you are ready to begin testing, open the sealed pouch by tearing along the notch. Remove the test from the pouch.
2. Immerse the strip into the container with the arrow end pointing towards the container. Do not immerse past the MAX (maximum) line.
Take the strip out after 8-10 seconds and lay the strip flat on a clean, dry, onabsorbent surface (e.g., mouth of the serum container).
3.Wait 10-15 minutes and read result. Do not read results after 20 minutes.
Cassette
1. Open a pouch containing a cassette, lay the cassette.
2. Using the plastic pipettor provided, draw about 2-3 drops (100mL) the sample into the sample well of the cassette.
3. Read results within 10-15 minutes. Do not read results after 20 minutes.
INTERPRETATION OF RESULTS
Negative: Only one pink band appears on test region of the Cassette. This indicates that there is no detectable Anti-Syphilis in the serum.
Positive: Two pink bands appear on test region of the Cassette. This indicates that the specimen contains detectable amount of Anti-Syphilis.
Invalid: If without colored band appears on test region, this is an indication of a possible error in performing the test. The test should be repeated using a new device.
Syphilis Test PRECAUTION:
1. Must use fresh specimen and avoid repetitive freezing, the result will be invalid
2. Use it before expiry date.
3.The package of kit should not be opened until it reaches the room temperature if it taken out from the refrigerator.
4 .Old Serum can not be used. If the serum is thick, it can be used only after being separated.
The One Step HAV IgG/IgM Test is a rapid chromatographic immunoassay for the qualitative detection of antibodies (IgG and IgM) to Hepatitis A Virus (HAV) in Whole Blood /Serum / Plasma to aid in the diagnosis of Hepatitis A Virus.
Summary
Hepatitis A is an acute, usually self-limiting disease of the liver caused by hepatitis A virus (HAV). HAV is transmitted from person to person, primarily by the faecal-oral route. The incidence of hepatitis A is closely related to socioeconomic development, and seroepidemiological studies show that prevalence of anti-HAV antibodies in the general population varies from 15% to close to 100% in different parts of the world. One step HAV IgG/IgM Test is a simple, visual qualitative test that detects Hepatitis A Virus antibodies in human Whole Blood /Serum / Plasma. The test is based on immunochromatography and can give a result within 15 minutes.
Principle
The One Step HAV IgG/IgM Test is a qualitative membrane strip based immunoassay for the detection of Hepatitis A Virus antibodies (IgG and IgM) in Whole Blood /Serum / Plasma. The test device consists of: 1) a burgundy colored conjugate pad containing HAV recombinant envelope antigens conjugated with Colloid gold (HAV conjugates) and rabbit IgG-gold conjugates,2) a nitrocellulose membrane strip containing two test bands (T1 and T2 bands) and a control band (C band). The T1 band is pre-coated with the antibody for the detection of IgM anti-HAV, T2 band is coated with antibody for the detection of IgG anti-HAV, and the C band is pre-coated with goat anti rabbit IgG. When an adequate volume of test specimen is dispensed into the sample well of the test cassette, the specimen migrates by capillary action across the cassette. IgG anti-HAV, if present in the specimen, will bind to the HAV conjugates. The immunocomplex is then captured by the reagent pre-coated on the T2 band, forming a burgundy colored T2 band, indicating a HAV IgG positive test result and suggesting a recent or repeat infection. IgM anti-HAV if present in the specimen will bind to the HAV conjugates. The immunocomplex is then captured by the reagent coated on the T1 band, forming a burgundy colored T1 band, indicating a HAV IgM positive test result and suggesting a fresh infection. Absence of any T bands (T1 and T2) suggests a negative result. The test contains an internal control (C band) which should exhibit a burgundy colored band of the immunocomplex of goat anti rabbit IgG/rabbit IgG-gold conjugate regardless of the color development on any of the T bands. Otherwise, the test result is invalid and the specimen must be retested with another device.
Storage and Stability
Store as packaged in the sealed pouch at room temperature or refrigerated (4-30 or 40-86). The test device is stable through the expiration date printed on the sealed pouch.
The test must remain in the sealed pouch until use.
The ultra pure 97-kda recombinant single polypeptide taq dna polymerase of thermusaquaticus, expressed and purified from e.Coli. Recombinant polypeptide taq dna polymerase is ideal for standard pcr of amplification of 5kb and shorter fragment
Taq dna polymerase (5u/ul)along with 10x standard taq buffer in two categories 500u and 1000u
