What is PIPES buffer
PIPES Buffer (CAS5625-37-6) chemical name is 1,4-piperazine diethanesulfonic acid, which is a zwitterionic biological buffer. It is a white powder solid at room temperature. PIPES Buffer is not easily soluble in water (1g/L (100C). It can be dissolved by mixing equimolar sodium salt solution and titrating to the appropriate pH value. For example, PIPES Buffer (CAS76836-02-7) and PIPES sesquisodium salt ( CAS100037-69-2).
Application of PIPES Buffer
PIPES Buffer is widely used in cell culture, chromatography, cosmetics, diagnostic testing, protein purification and electron microscopy research. PIPES Buffer is similar to MES Buffer and MOPS Buffer (also produced by Desheng). This Good's Buffer lacks the ability to form complexes with most metal ions, so it is recommended to be used as a non-coordinating buffer in metal ion solutions.
Advantages of PIPES Buffer
According to related literature, the use of NaOH-PIPES buffered glutaraldehyde solution in the ultrastructure of various tissues, such as human skin, human hypertrophic scars, mollusk neuron tissue, etc., the results show that more organelles and organelles can be observed Between the details. The cytoplasm is more uniform in consistency. Compared with NaOH-PIPES buffer, microfilaments and microtubules are more common in PIPES tissues. In addition, PIPES Buffer is proven to be comparable in living cells.
What is TRICINE buffer
TRICINE Buffer (CAS 5704-04-1), a zwitterionic biological buffer containing amine and acid groups. This product is a white crystalline powder, slightly soluble in water (the solubility is 1.8g/100ml water).
Application of TRICINE Buffer
TRICINE is widely used in cell culture and electrophoresis research. As a buffer for cell culture research, TRICINE can inhibit the unexpected growth of mycoplasma in animal tissue culture. It has also been used in bacterial culture to prevent precipitation of iron salts. The Good's buffer can form complexes with most metal ions, such as Mg, Ca, Co, Cu, Ni, and Zn, so the stability constant and concentration should be considered when choosing the buffer. As a buffer for electrophoresis research, Tricine has been used as an alternative to protocols using the controlled substance barbital.
Advantages of TRICINE Buffer
TRICINE has a higher negative (more negative) charge than glycine, making it migrate faster. In addition, its high ionic strength results in more ion movement and less protein movement, so that low-molecular-weight proteins can be separated in a lower percentage of acrylamide gels. Tricine has been proven to be used to separate proteins in the range of 1 to 100 kDa by electrophoresis. In ten tests using firefly luciferase for ATP determination, 25mmol/L tricine buffer was found to be the most effective buffer.
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KS-JIS Cast Iron Buffer is a valve accessory that conforms to the Korean and Japanese Industrial Standard (KS-JIS) specifications and is mainly used in industrial fluid piping systems to mitigate shocks and vibrations in the piping system and to protect valves and equipment from damage. Made of cast iron material, this snubber is characterized by corrosion resistance and sturdy construction, and is suitable for use in various fluid control systems.
Buffer solution is a type of buffer system dedicated to life science research. In biochemical experiments, buffer solution plays an indispensable role. It can resist the influence of a small amount of strong acid and alkali from the outside, and maintain the system closest to the physiological environment. pH value. Both HEPES and PIPES buffers are commonly used buffers in biological experiments.
The pH buffer range of HEPES is 6.8-8.2. It is a zwitterionic biological buffer. It is soluble in water and does not form stable complexes with metal ions. In most cases, it will not interfere with biochemical processes and can be widely used in a variety of Biochemical reactions and used as buffer reagents in certain cell culture media. HEPES is often used in the cell culture medium of various types of organisms; in protein research, PIPES is often used as the component and eluent of the binding buffer in cation exchange chromatography; in DNA research, PIPES is used as calcium phosphate and DNA The buffer for the precipitation formation system, AFM and the buffer for electroporation experiments. In addition, HEPES interferes with the reaction between DNA and restriction enzymes, and is not suitable for Lowry's method to determine protein content. HEPES buffer is often used in the research of organelles and highly variable, pH-sensitive proteins and enzymes, as well as in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. It is a hydrogen ion buffer that can control a constant pH range for a long time. The final concentration is 10-50mmol/L, and the buffering capacity can be achieved when the culture medium contains 20mmol/L HEPES.
The pH buffer range of PIPES is 6.1-7.5, insoluble in water, soluble in NaOH aqueous solution. PIPES is different from buffers containing two (2-hydroxyethyl) amino groups (such as Bis-tris, Bicine), and can not form stable complexes with most metal ions. It is suitable for buffers in solution systems containing metal ions. According to existing research results, PIPES can be used to purify tubulin using phosphocellulose chromatography, to purify recombinant GTP-binding proteins ARF1 and ARF2 by gel filtration, and to use as a buffer to crystallize transketolase from E. coli. In addition, because PIPES can form free radicals, it is not suitable for redox systems. In cation exchange chromatography, a low concentration of PIPES buffer should be used, because PIPES has a relatively large ionic strength and its pKa value is concentration-dependent.
We must learn to distinguish between the similarities and differences in these reagents, so as to better promote the development and production of our different products. Desheng unanimously adheres to this kind of subtle difference, so that it can continue to develop and produce better products.
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Introduction:
4-hydroxyethyl piperazine ethosulfonic acid (HEPES) is an important hydrogen ion buffer, which has a good buffer ability in the pH range of 6.8 to 8.2, and can control a constant pH for a long time. Using a concentration of 10 ~ 50mmol/L, the general culture medium containing 20mmol/L 4-hydroxyethyl piperazine taurine can have a good buffer capacity, and has no toxic effect on cells.
Application:
HEPES (4-hydroxyethyl piperazinethesulfonic acid) is a non-ionic amphoteric buffer with high polarity, and is inert to a variety of chemical reagents and enzymes. It does not participate in and interfere with the process of biochemical reactions, and has no inhibitory effect on enzyme chemical reactions. Therefore, it can be used specifically for the research of proteins and enzymes that are highly volatile and ph-sensitive to organelles and very volatile.
Buffer solution preparation:
(1)hepes buffer can be directly added to the prepared culture medium according to the desired concentration, and then filtered to remove bacteria. 2.38 g HEPES was added to each 1000ml of culture medium, the pH was adjusted to 7.2 with lNNaOH after dissolution, and the bacteria were removed by filtration before use. At this time, the concentration of HEPES was 10mmol/L.
(2) 100x storage solution (lmol/L) could also be prepared. Before use, 99ml culture solution was added to lml storage solution, and the final concentration was 10mmol/L. lmol/L (100x) hepes buffer was prepared as follows: 23.8gHEPES was dissolved in 90ml double distilled water, the pH was adjusted to 7.5-8.0 with lNNaOH, and then the water was fixed to 100ml, filtered to remove bacteria, divided into vials (2ml/ bottle), and stored at 4�ºC or -20�ºC.
Purpose:
1.Biological buffer; Reaction buffer, prehybridization buffer and hybridization buffer for isolation and analysis of RNA nuclear fraction; For RNA with T4RNA
2.Molecular biology grade is used for RNA3' -end labeling with T4 RNA ligase
3.Cell-cell adhesion, short-term cell aggregation culture, cleaning of tissue and cell buffer
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Introduction:
4-hydroxyethyl piperazine ethosulfonic acid (HEPES) is an important hydrogen ion buffer, which has a good buffer ability in the pH range of 6.8 to 8.2, and can control a constant pH for a long time. Using a concentration of 10 ~ 50mmol/L, the general culture medium containing 20mmol/L 4-hydroxyethyl piperazine taurine can have a good buffer capacity, and has no toxic effect on cells.
Application:
HEPES (4-hydroxyethyl piperazinethesulfonic acid) is a non-ionic amphoteric buffer with high polarity, and is inert to a variety of chemical reagents and enzymes. It does not participate in and interfere with the process of biochemical reactions, and has no inhibitory effect on enzyme chemical reactions. Therefore, it can be used specifically for the research of proteins and enzymes that are highly volatile and ph-sensitive to organelles and very volatile.
Buffer solution preparation:
(1)hepes buffer can be directly added to the prepared culture medium according to the desired concentration, and then filtered to remove bacteria. 2.38 g HEPES was added to each 1000ml of culture medium, the pH was adjusted to 7.2 with lNNaOH after dissolution, and the bacteria were removed by filtration before use. At this time, the concentration of HEPES was 10mmol/L.
(2) 100x storage solution (lmol/L) could also be prepared. Before use, 99ml culture solution was added to lml storage solution, and the final concentration was 10mmol/L. lmol/L (100x) hepes buffer was prepared as follows: 23.8gHEPES was dissolved in 90ml double distilled water, the pH was adjusted to 7.5-8.0 with lNNaOH, and then the water was fixed to 100ml, filtered to remove bacteria, divided into vials (2ml/ bottle), and stored at 4�??�?�ºC or -20�??�?�ºC.
Purpose:
1.Biological buffer; Reaction buffer, prehybridization buffer and hybridization buffer for isolation and analysis of RNA nuclear fraction; For RNA with T4RNA
2.Molecular biology grade is used for RNA3' -end labeling with T4 RNA ligase
3.Cell-cell adhesion, short-term cell aggregation culture, cleaning of tissue and cell buffer
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