Hiv Antigen

Product Details:

Number of Reactions(Preps)/Kit : 96 Wells
Brand : CTK BioTech
Sample : serum / plasma
Storage : 2-8 C
Cat No : AE0420
INTENDED USE:

The TRUSTwell HIV Ag-Ab ELISA Kit is a solid phase enzyme linked immunosorbent assay for the qualitative detection of HIV-1 P24 antigen, anti-HIV-1 including subtype O and anti-HIV-2 antibodies (including isotype IgG, IgM and IgA) in human serum or plasma. It is intended for professional use only as an aid in the early identification of infection with HIV-1 and HIV-2 viruses. Any reactive specimen with the TRUSTwell HIV Ag-Ab ELISA Kit must be confirmed with alternative testing method(s) and clinical findings.

TEST PRINCIPLE:

The TRUSTwell HIV Ag-Ab ELISA Kit is a solid phase enzyme linked immunosorbent assay based on the principle of the double antibody/antigen sandwich technique for the detection of HIV-1 P24 antigen and various antibodies against HIV-1 and/or HIV-2 antigen in human serum or plasma. The TRUSTwell HIV Ag-Ab ELISA Kit is composed by two systems: 1) Solid microwells pre-coated with Recombinant HIV-1 and HIV-2 antigens and anti-P24 antibody 2) Liquid conjugates composed of a. Recombinant HIV-1 and HIV-2 antigens conjugated with horse radish peroxidase (HRP-HIV 1+2 conjugates) and avidin conjugated with HRP (HRP-avidin conjugates) b. Anti-P24 antibody conjugated with biotin (Biotinylated P24 Ab)

During the assay, the test specimen is first incubated with the coated microwells and followed by incubation with biotinylated P24 Ab. The anti-HIV-1 and anti-HIV-2 antibodies, if present in the specimen, bind to the antigens coated on the microwell surface. If P24 present in the specimen, binds to the anti-P24 antibody on the microwell surface and biotinylated P24 Ab in the solution. In the next incubation with the HRP-HIV 1+2 and HRP-avidin conjugates, the above bounded anti-HIV-1 and anti-HIV-2 antibodies and bounded P24 antigen reactive to the HRP conjugates, forming HRP-complex. Unbounded conjugates are then removed by washing. The presence of the complexed conjugates is shown by a blue color upon additional incubation with TMB substrate. The reaction is stopped with Stop Solution and absorbance are read using a spectrophotometer at 450/620-690nm.

Clinical Performance:

A total of 1100 patient specimens from susceptible subjects were tested by the TRUSTwell HIV Ag-Ab ELISA Kit.

Relative Sensitivity: 100%,
Relative Specificity: 99.9%,
Overall Agreement: 99.9%.

Uncut Sheet HIV 1/2 Tri-Lines Rapid Test

Package:30sheets/pouch

Specimen:Whole blood/Serum/plasma

INTENDED USE

The HIV-1/2 Rapid Screen Test (RST) is a qualitative test for the detection of antibodies to Human Immunodeficiency Virus type 1 and 2(HIV-1/2) in human serum/plasma and whole blood. It is considered as an initial screening test for HIV-1/2 antibodies. All positive specimens must be confirmed with Western Blot or other qualified EIA.

PRECAUTION

1.For in vitro diagnostic uses only.

2.All patient samples should be treated as if capable of transmitting diseases.

3.Do not use test kit beyond expiration date.

4.Icteric, lipemic, hemolysed, heat treated and contaminated sera may cause erroneous results.

SUMMARY AND EXPLANATION OF THE TEST

It has been shown that the acquired immunodeficiency syndrome (AIDS) is caused by viruses transmitted by sexual contact, transfusion, use of contaminated blood products and sharing contaminated needles. HIV-1 and HIV-2 viruses have been isolated from patients with AIDS and AIDS-related complex (ARC), high-risk persons for AIDS. HIV-1 and HIV-2 viruses delete T helper cells, a subpopulation of T cells for body defense, thus causing AIDS patients susceptible to opportunistic infections and developing malignant tumors. The incidence of specific antibodies to HIV-1/2 is high in AIDS, ARC and persons with high risk for AIDS. The HIV-1/2 Rapid Screen Test is designed to detect antibodies to HIV-1/2 in AIDS patients, ARC or high risk persons and identify any potential donors carrying these antibodies in serum specimens.

PRINCIPLE

HIV-1/2 RST is a chromatographic immunoassay (CIA) for the detection of antibodies to HIV-1/2 in human serum/plasma and whole blood. HIV-1/2 specific antigens are precoated onto membrane as a capture reagent on the test region. During the test, specimen is allowed to react with the colloidal gold particles, which have been labeled with HIV-1/2 specific antigens. Antibodies to HIV-1/2, if present, a red colored band will develop on the membrane in proportion to the amount of HIV-1/2 antibodies present in the specimen. Absence of this red colored band in the test region suggests a negative result. To serve as a procedural control, red colored band in the control region will always appear regardless the presence of antibodies to HIV-1/2.

One step anti-hiv rapid test

One step anti-hiv test is a rapid direct binding screening test for the presence of antibodies to hiv1and hiv2 viruses.The test is based on the principle of double antigen sandwich immunoassay for detection of anti-hiv in serum.Purified recombinant antigens are employed to identify anti-hiv specifically.

Product Details:

Number of Reactions(Preps)/Kit : 96 Wells
Brand : CTK BioTech
Sample : serum / plasma
Stroage : 2-8 C
Cat No : AE0410
INTENDED USE:

The TRUSTwell HIV 1+2 Ab ELISA Kit is a solid phase enzyme linked immunosorbent assay for the qualitative detection of anti-HIV-1 including subtype O and anti-HIV-2 antibodies (including isotype IgG, IgM and IgA) in human serum or plasma. It is intended for professional use only as an aid in the diagnosis of infection with HIV-1 and HIV-2 viruses. Any reactive specimen with the TRUSTwell HIV 1+2 Ab ELISA Kit must be confirmed with alternative testing method(s) and clinical findings.

TEST PRINCIPLE :

TRUSTwell HIV 1+2 Ab ELISA Kit is a solid phase enzyme linked immunosorbent assay based on the principle of the double antigen-sandwich technique for the detection of the various antibodies against HIV-1 and/or HIV-2 in human serum or plasma. The TRUSTwell HIV 1+2 Ab ELISA Kit is composed of two key components: 1) Solid microwells pre-coated with recombinant HIV-1 and HIV-2 antigens; 2) Liquid conjugates composed of recombinant HIV-1 and HIV-2 antigens conjugated with horse radish peroxidase (HIV 1+2 HRP conjugates). During the assay, the test specimen is first incubated with the coated microwells. The anti-HIV-1 and anti-HIV-2 antibodies, if present in the specimen, bind to the antigens coated on the microwell surface. In the second incubation with the HRP- HIV 1+2 conjugates, the anti-HIV-1 and antiHIV-1 antibodies absorbed on the surface of microwell react to the HRP-HIV 1+2 conjugates. Unbounded conjugates are then removed by washing. The presence of the complexed conjugates is shown by a blue color upon additional incubation with TMB substrate. The reaction is stopped with Stop Solution and absorbance are read using a spectrophotometer at 450 /620-690 nm.

Clinical Performance :

A total of 1095 patient specimens from susceptible subjects were tested by the TRUSTwell HIV 1+2 Ab ELISA Kit.

Relative Sensitivity: 100% Relative Specificity: 99.9%, Overall Agreement: 99.9%

Usage/Application Hospital
Type Rapid Test Kit
Results 3 mins
Packaging Size 100 Tests
Country of Origin Made in India

The HIV TRI-DOT test is a visual, rapid, sensitive and accurate immunoassay for the differential detection of HIV-1 & HIV-2 antibodies (IgG) in human serum or plasma using HIV-1 & HIV-2 Antigens immobilized on an immunofiltration membrane. The test is a screening test for Anti HIV-1 and HIV-2 and is for in vitro diagnostic use only.
SALIENT FEATURES
Rapid visual test ,based on Flow Through Technology.
Differential detection of HIV-1 & HIV-2.
Detection of group O & subtype C
Use of envelop antigens gp41& C terminus of gp120 for HIV-1 & gp36 for HIV-2.
100% sensitivity & 100% specificity as per WHO Evaluation.
Shelf life 15 months at 2-8�°C
Available in convenient pack sizes-10 Tests, 50 Tests and 100 Tests.
Results within 3 minutes.
No instrument required.
In built quality control dot.

Product Details:

Number of Reactions(Preps)/Kit 100
Brand Alere
Usage/Application Hospital
Result Time (Rapid Kits) 15 mins
The irst and only FDA approved rapid point-of-care test that simultaneously andseparately detects HIV-1/2 antibodies and free HIV-1 p24 antigen on a single test strip.Determine Combo
Pack Size : 100 Test
Brand : 100 Test

Product Details:

Brand J.Mithra
Kit Type HIV Test Kit (Rapid) (Tri Dot)
Sensitivity 100% WHO Evaluation
Specificity 100% WHO Evaluation
Result Time Within 3 Minutes
Cat. No IR130100
Intended Use:The HIV TRI-DOT test is a visual, rapid, sensitive and accurate immunoassay for the differential detection of HIV-1 & HIV-2 antibodies (IgG,IgM & IgA) in human serum or plasma using HIV-1 & HIV-2 Antigens immobilized on an immunofiltration membrane. The test is a screening test for Anti HIV-1 and HIV-2 and is for in vitro diagnostic use only.

Salient Features:
Rapid visual test ,based on Flow Through Technology.
Differential detection of HIV-1 & HIV-2.
Detection of group O & subtype C.
Use of envelop antigens gp41& C terminus of gp120 for HIV-1 & gp36 for HIV-2.
100% sensitivity & 100% specificity as per WHO Evaluation.
Shelf life 24 months at 2-8C
Available in convenient pack sizes- 50 Tests and 100 Tests.
Results within 3 minutes.
No instrument required.
In built quality control dot.
Evaluations:

WHO Evaluation: Evaluation report from UNAIDS (WHO), CH-1211Geneva,Switzerland Aug 1999 the Sensitivity and Specificity of HIV TRI-DOT is 100 % .The panel used for evaluation of HIV TRI-DOT by Institute of Tropical Medicine, WHO Collaborating Centre in AIDS, Belgium also included HIV-O virus, which was found reactive with HIV TRI-DOT.
NIMHANS BANGALORE: Evaluation report from National HIV Reference Laboratories of Government of India , claiming Sensitivity and Specificity of HIV TRI-DOT to be 100 %.
CMC VELLORE: Evaluated by Department of Clinical Virology, Christian Medical College & Hospital, Vellore and published in JCM, vol. 38, Issue 2000,claiming 99.5% sensitivity and 99.9% specificity.
NARI PUNE: Evaluation Report from National AIDS Research Institute(NARI-Pune) claiming sensitivity & Specificity to be 100 %.
Approved by Drug Controller General of India( DCGI) for the usage in Blood Banks.