Nucleic Acid Extraction Kit

AllMagTM Nucleic acid extraction kits

1. AllMagTM Forensic Kit
Description
AllMagTM Forensic Kit using our patent superparamagnetic beads and distinct buffer system can extract genomic DNA rapidly and sufficiently from the samples such as blood spot, saliva, mouth swab and hair root,. DNA separated by the Kit can apply for genotyping, sequencing and PCR.This kit is especially suitable for purification nucleic acid of trace amount, and can be operated automatically and manually.

2.AllMagTM PCR Purification Kit
Description
AllMagTM PCR Product Purification Kit can extract PCR product rapidly and sufficiently from PCR buffer, using our patent superparamagnetic beads and distinct buffer system. DNA purified by the Kit can apply for digesting of restricted enzyme, sequencing and ligating. The kit is especially suitable for purification of PCR product with high throughput and automatically. It can also be operated manually.
3.AllMagTM Blood Genomic DNA Kit
Description
AllMagTM Blood Genomic DNA Kit can extract genomic DNA rapidly and sufficiently from whole blood, using our patent superparamagnetic nanospheres and distinct buffer system. DNA separated by the Kit can apply for digesting of restricted enzyme, sequencing and transformation. The kit is especially suitable for purification of genomic DNA with high throughput and automatically. It can also be operated manually.

The Nucleic Acid Isolation Kit (Magnetic Beads Method) is designed for the automated purification of RNA and DNA from body fluids (such as swabs, plasma, serum) using automated nucleic acid extraction instruments. Magnetic- particle technology provides high-quality DNA/RNA that is suitable for direct use in downstream applications such as amplification or other enzymatic reactions.
We provide different models to match different machines, also, we can provide OEM service for customers

Magnetic bead-based purification 1. High-quality nucleic acid can be obtained range from 10 min to 40min (up to different protocol). 2. High throughput: Can be integrated with magnetic rod method automatic instrument or pipetting method automatic instrument to carry out high throughput extraction experiment. 3.Safe and nontoxic: No toxic organic reagents such as phenol/chloroform are needed. 4.Complete removal of contaminants and PCR inhibitors for downstream experiments.

CE Magnetic Bead Method Nucleic Acid Extraction and Purification Kit

It's designed for the rapid, efficient and automatic isolation and purification of high quality virus nucleic acid from a variety of samples on the automatic nucleic acid extraction system.

Features:
1): Rapid and Reliable: Fast procedures and easy to use
2): High Sensitivity
3): High Efficiency: â?¥ 95% of the nucleic acid can be combined.
4): Versatile: Nucleic acid of various virus, Hepatitis, etc. in different samples, such as Serum, Plasma, Whole blood, Swabs, Saliva, Body fluid, Tissue, Feces, BALF, etc. for a broad range of down stream applications.
5): Safe: Non-toxic

Specification: 50T/kit or 100T/kit vial; 48T/kit or 96T/kit 0.1mL 8 strips/0.2mL 8 strips.
Canine distemper virus(CDV) is caused by a single-stranded RNA virus of the family . The disease is highly contagious via inhalation. The distemper virus real time PCR detection kit is intended for the qualitative detection of Canine distemper virus in samples by using real time PCR systems.

RT-PCR direct Detection Kit is intended to be used to achieve direct detection of Severe acute respiratory syndrome coronavirus 2 viral RNA from nasal swabs, nasopharyngeal swabs and oropharyngeal swabs from patients with signs and symptoms of infection who are suspected of COVID-19. This product provides dual-detections of two independent genes of a single tube. gene and N gene is amplified and detected in FAM and HEX channel, respectively. An internal control is set with a Cy5 labeled probe using the RNase P gene of human as the target, to monitor the amplification effect of the detection system.
Our nucleic acid detection reagent can be transported at room temperature by freeze-drying technology, and can be stored at low temperature for a long time for one and a half years, and maintain good effectiveness.

Description

This kit utilizes fluorescence quantitative probe-based PCR and guarantees a high specificity to ensure accurate one-step identification of ORF1ab and N genes of 2019-nCoV. The kit offers a highly sensitive test with a limit of detection as low as 500 copies/ml. The results are available within 1.5 hour.

Kit Contents (48 Tests /Kit)
Dual Enzyme solution A 50 µl
Dual Ncvo-O / N reaction solution A 1 ml
Dual Ncvo-O / N positive control A 200 µl
Dual Negative control A 200µl

Applicable equipment
ABI series, Bio-Rad, Roche series and other fluorescent real-time PCR instruments.

Specimens requirements
Specimen type: nasopharyngeal swab from suspected infection; virus cell culture fluid, etc.

Basic Protocol
1. Sample preparation.
Take the test sample and extract the RNA nucleic acid according to the instructions of the nucleic acid extraction kit. The nucleic acid extraction product should be stored at -20 C.
2. Reaction mixture preparation
According to the total number of test samples, the number of PCR reaction tubes needed is. N = number of samples + 1 negative control + 1 positive control.
The following protocol is recommended for a 20 µl reaction volume. If the volume of reaction changes, please adjust proportionally.

Components Volume
Dual Enzyme solution A 1 µl
Dual Ncvo-O / N reaction solution A 19µl
Final Volume: 20 µl

3. Sample adding.
Add 5 µl each of the extracted RNA, positive and of negative control to corresponding reaction tubes. After assembling all the components, cover the tube caps and gently mix the contents of the tube, mix well, and centrifuge briefly.

4. Perform quantitative PCR
Place the reaction tube inside a real-time PCR instrument.
Set the channel and sample information, reaction system volume 25 µl.
Select the following channels: FAM channel for nCOV-ORF1ab, VIC channel for nCOV-N.
Perform quantitative PCR using recommended cycling parameters settings:

Step Temperature Time Number of cycles Fluorescence Detector
1 50C 15 min 1 Off
2 95C 3 min 1 Off
3 95C 10sec 45 Off
60C 30 sec On
Result analysis:
1) Set the baseline: Generally, it is set to 6-15cycle for ABI 7500, 7700 and other instruments, 3-15cycle for PE5700, and 6-12cycle for MJ Research Option2. Under special circumstances, the baseline can be adjusted accordantly.
2) Set the threshold: The threshold line just exceeds the highest point of the negative control amplification curve (random noise line).