Since the outbreak of the epidemic, relevant diagnostic reagent R&D and production companies have introduced RT-PCR nucleic acid detection kits for the first time. By collecting oropharyngeal swab or nasopharyngeal swab samples, the RNA of the virus is extracted and purified for qualitative or quantitative detection. "2019 -New Coronavirus". The sample collection and storage container used in this method is a "disposable virus sampling tube". The new coronavirus nucleic acid detection, disposable virus sampling tube can be said to be indispensable. Next, Desheng intends to introduce the composition and use of the virus sampling tube.
About the composition and manufacturing of the virus sampling tube:
1. Sampling swab: The sampling swab directly contacts the sampling site, and the material of the sampling head is closely related to the subsequent detection.
2. Virus preservation solution: There are two main types of virus preservation solution widely used in the market. One is the improved virus maintenance solution based on the delivery medium, and the other is the improved preservation solution of nucleic acid extraction lysis solution.
3. Preservation tube: The material of the preservation tube should be selected carefully. There are data indicating that Polypropylene is related to the adsorption of nucleic acids, especially when the ion concentration of high tension is high, polyethylene plastic (Polyethylene) is better than polypropylene (Polypropylene). Easy to grasp DNA/RNA.
About the use of virus sampling tube:
The virus sampling tube sampling is mainly divided into oropharyngeal sampling and nasopharyngeal sampling:
1. Oropharyngeal sampling: first press the tongue with a tongue depressor, and then put the sampling swab head into the throat to wipe the bilateral pharyngeal tonsils and back wall of the pharynx, wipe the back wall of the pharynx with light force, avoid touching the tongue unit.
2. Nasopharyngeal sampling: use a swab to measure the distance from the tip of the nose to the earlobe and mark it with your fingers. Insert the sampling swab into the nasal cavity in the direction perpendicular to the nose (face). The distance of the swab should be at least half the length of the earlobe to the tip of the nose. Leave the swab in the nose for 15-30 seconds, gently rotate it 3-5 times, and withdraw the swab.
It is not difficult to see from the method of use that whether it is an oropharyngeal swab or a nasopharyngeal swab, sampling is a technical task, which is difficult and easy to contaminate. The quality of the collected sample is directly related to the subsequent testing. If the viral load of the collected sample is Low, easy to cause false negatives, difficult to diagnose.
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